Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIYGIYMDRPLSQEENERFMSFISPEKREKCRRFYHKEDAHRTLLGDVLVRSVISRQYQ------LDKSDIRFSTQEYGKPCIPDL----PDAHFNISHSGRWVICAFDSQPIGIDIEKTKPISLEIAKRFFSKTEYSDLLAKDKDEQTDYFYHLWSMKESFIKQEGKGLSLPLDSFSVRLHQDGQVSIELPDSHSPCYIKTYEVDPGYKMAVCAAHPDFPEDITMVSYEELL 1F7L Chain:A ((23-115)) ----------------KRFAERILTRSELDQYYELS-EKRKNEFLAGRFAAKEAFSKAFGTGIGRQLSFQDIEIRKDQNGKPYIICTKLSPAAVHVSITHTKEYAAAQVV----------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1F7L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 303 -42951 -141.75 -517.48 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -141.75 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.462

(partial model without unconserved sides chains): PDB file : Tito_1F7L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1F7L-query.scw PDB file : Tito_Scwrl_1F7L.pdb: