Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------MHVITTQV-----LF-IFCFLLLIHSIETLAYATRLSGARVGFI-ASALSLFNVMVIVSRMSNMVQQPFTGHLID--------DAGKNALAIV------------GEQFRFLIFGSTV-GTILGIILL---PSFVA-LFSRAIIHLAGGGGSVFQVFRKG--------FSKQGFKNALSYL------RLPSISY-VKGFHMRLIP----KRLFVINM-LITSIYTIGVLS-ALYAGLLAPERSTTAVMASGLINGIATMLLAIFVDPKVSV-LADDVAKGKRSYIYLKWTSVTMVTSRVAGTLL-------AQLMFIPGAYY-I-AWLTKWF---------------------------------- 4MTN Chain:A ((5-406)) GNEVLRIVDSIHRDKSIDKEIVFEGVEQAILSAARKHFGEEEVIEVHIDRTSGQPMVKTNGREIDRDELGDILGRIS-AQTKQVMIQKIREAERDTLFDEYAQLRGQIVSGTVTRNEGSAITVNIGKAEAILPRSEMIPGESHRPNERIRAVVLEVKKMGPRVRVVLSRAHPDFVRRLLELEIPEVNERIIEIRSLAREAGYRTKVAVSCADSNIDPVGACVGVRGARIRNVGEELGGERIEVVRWNDSLQVLVPNAMQPSEVEDVILCPMLGRVLVLVRDDQLSLAIGK---RGQNVRLASKLVGWDIDVMTREELDQQLDQAVV-AYSQIPGVSEELAEGLVSQGFLSFEDLSVIEPDELMEMGSLTQEQADVIVEYAERESERIEKEQDLRRATEKAERQSQE

General information: TITO was launched using: RESULT: Template: 4MTN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 879 -159218 -181.13 -603.10 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -181.13 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.131

(partial model without unconserved sides chains): PDB file : Tito_4MTN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MTN-query.scw PDB file : Tito_Scwrl_4MTN.pdb: