Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHQVRCAVLGLGRLGYYHAKNLVTSVPGAKLVCVGDPLKGRAEQVARELGI-EKWSEDPYEVLEDPGIDAVIIVTPTSTHGDMIIKAAENGKQIFVEKPLTLSLEESKAASEKV-KETGVICQVGFMRRFDPAYADAKRRIDAGEIGKPIYYKGFTRDQGAPPAEFIKHSGGIFIDCSIHDYDIARYLLGAEITSVSGHGRILNNPFMEQYGDVDQALTYIEFDSGAAGDVEASRTSPYGHD-IRAEVIGTEGS-IFIGTLRHQHVTILSAKGSSFDIIPDFQTRFHEAYCLELQHFAECVRNGKTPI-VTDIDATINLEVGIAATNSFRNGMPVQLDVKRAYTGM 4MIY Chain:A ((1-333)) --MVVKVGVIGTGAMGRAHIDRLTNVLTGAEVVAVTDIDHEAAEAAVRDFHLNAKVYPDDTSLLQDPDIDAVFVVSFGGAHEATVLKALDTDKFIFTEKPLATTLEGAKRIVDKELTKSKKVIQVGFMRRYDQGIRALKEKLDTGIIGAPLVVRASHINPN-VASN--YSNEMAITDTLIHEIDEMHWLLDDEYTSIQITYPRQSA-EVRNEGLHDPQLATLTTKKGTVIQVLVHVTAQYG-YEVKLEVIGETGELQLPNYGL--GPILRSNANQQTAVEMSWINRFIQAYNTEVQEFIDEVAKSEPPVGPSAWDGYIAAITAAAANRSQKDQETVLINVAG-----

General information: TITO was launched using: RESULT: Template: 4MIY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1755 -153436 -87.43 -467.79 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -87.43 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.423

(partial model without unconserved sides chains): PDB file : Tito_4MIY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MIY-query.scw PDB file : Tito_Scwrl_4MIY.pdb: