Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEVKTISVEDTYEIRH---RILRPH---QSIEQCKYKED-HAEGSFHLGVFYDGTLISIASFSPQSQPL---------LTEASAYRLRGMATLEGYRDQKAGSTLIKHAEHKLAES-GVQAVWCNARHHVKGYYAKLGWKELGEPFD-IPGIGNHIVMYKTLRTSR 1KUX Chain:A ((35-195)) -NEFRCLTPEDAAGVFEIEREAFISVSGNCPLNLDEVQHFLTLCPELSLGWFVEGRLVAFIIGSLWDEERLTQESLALHRPRGHSAHLHALAVHRSFRQQGKGSVLLWRYLHHVGAQPAVRRAVLMCEDALVPFYQRFGFHPAGPCAIVVGSLT-FTEMHCSL----

General information: TITO was launched using: RESULT: Template: 1KUX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 649 -102571 -158.04 -717.28 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -158.04 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.427

(partial model without unconserved sides chains): PDB file : Tito_1KUX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KUX-query.scw PDB file : Tito_Scwrl_1KUX.pdb: