Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIAKVGNVIEFKNGLTGVVEKVNENSVIVDVTIMDNYRDLELDSLTVVNHKNYKIIRDSY 2QF4 Chain:A ((35-68)) ---ASENMLAIANGGLIGSVSKVEENSTIVNLLTNTE------------------------

General information: TITO was launched using: RESULT: Template: 2QF4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 79 -10656 -134.89 -313.41 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -134.89 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.604

(partial model without unconserved sides chains): PDB file : Tito_2QF4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QF4-query.scw PDB file : Tito_Scwrl_2QF4.pdb: