Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNIGEI-ISNFEGIIGALLGVIVTLILTHILKHFGQIKFYIVDF-EIYFKTDNDGWG--TNVMPSKDEAKQIEIHSQIEIYNGAEIPK-VLREIKFCFYKNTNLIVSVTPDDKATTEE--FAEFGYYRDKLFNINL-PSK-QIIAINIIKF-LNEKETKQVKKCNRVYLEAKDHNGKMYKVFLGEF 1V0A Chain:A ((3-175)) -SAVGEKMLDDFEGVLNWGSYSGEGAKVSTKIVSGKT-----GNGMEVSYTGTTDGYWGTVYSLPDGDWSKWLKISFDIKS-VANEIRFMIAEKSINGVGDGEHWVYSITPDSSWKTIEIPFSSFRRRLDYQPPGQDMSGTLDLDNIDSIHFMYANNKSGKFVVDNIKLIGALEHHH----------

General information: TITO was launched using: RESULT: Template: 1V0A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 828 46646 56.34 291.53 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 56.34 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.163

(partial model without unconserved sides chains): PDB file : Tito_1V0A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V0A-query.scw PDB file : Tito_Scwrl_1V0A.pdb: