Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKKALIFTVIFGIIFLAVLLVSASIYKSAMAQKEEGHEAAAAEAKKETDLAHVDQVETFVGKEKYYVVKGTDKKGTALYVWVPADKKAKILSKEAKEGISEDKAAKIIKDEGL-------VSKQKEVHLAREG-----NVLLWEVTYLDK---EGQYSLSYVDFTTGKILKNITP 3NQZ Chain:A ((97-175)) ----------------------------------------------------------------------------------------------DVSAKLTKKDAILKGSKTGIAAKSVGLKKHNEQSRLAIWVDDQNKAHLVYEVSYVTYGKSPSRPY-LIIDANTGEVLLSY--

General information: TITO was launched using: RESULT: Template: 3NQZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 169 -2198 -13.00 -34.34 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -13.00 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.654

(partial model without unconserved sides chains): PDB file : Tito_3NQZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3NQZ-query.scw PDB file : Tito_Scwrl_3NQZ.pdb: