Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSLEEKTIAKEQIFSGKVIDLYVEDVELPNGKASKREIVKH-------PGAVAVLAVTDEG----KIIMVKQFRKPLERTIVEIPAGKLEKGEEPEYTALRELEEETGYTAKKLTKITAFYTSPGFADEIVHVFL----AEELSVLEEKRELDEDEFVEVMEVTLEDALKLVESREVYDAKTAYAIQYLQLKEALQAQK 3L85 Chain:B ((5-171)) --------ISEELISEGKWVKLEKTTYMDPTGKTRTWESVKRTTRKEQTADGVAVIPVLQRTLHYECIVLVKQFRPPMGGYCIEFPAGLIDDGETPEAAALRELEEETGYKGDIAECSPAVCMDPGLSNCTIHIVTVTINGDDAENARPKPKPGDGEFVEVISLPKNDLLQRLDA-------------------------

General information: TITO was launched using: RESULT: Template: 3L85.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 632 39829 63.02 262.03 target 2D structure prediction score : 0.34 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : 63.02 2D Compatibility (Sec. Struct. Predict.) : 0.34 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.397

(partial model without unconserved sides chains): PDB file : Tito_3L85.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3L85-query.scw PDB file : Tito_Scwrl_3L85.pdb: