Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLLERLNQDMKLYMKNREKDKLTVVRMVKASLQNEAIKLKKDSLTEDEELTVLSRELKQ--RKDSLQEFSNANRLDLVDKVQKELDILEVYLPEQLS-EEELRTIVNETIAEVGAS-------SKADMGKVMGAIMPKVKGKADGSLINKLVSSQLS 3IP4 Chain:B ((362-473)) ------------------------------------------TKLTPENLAGMIKLIEDGTMSSKIAKKVFP----ELAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQIMKASKGQANPQLVNQLLKQELD

General information: TITO was launched using: RESULT: Template: 3IP4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 317 53423 168.53 523.75 target 2D structure prediction score : 0.83 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : 168.53 2D Compatibility (Sec. Struct. Predict.) : 0.83 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_3IP4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3IP4-query.scw PDB file : Tito_Scwrl_3IP4.pdb: