Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MNNE------PVKRGKKNRWELNLPIMTYVVADDWIDKLGRETFTLWLRFHTWVDREDELRDYDRIPRSFE--NIYKKTLGISKSK-FYRLIKPLWEYGLIDIIEYEESNRNSTKPKNIIVYEYPLHEIERKYKPLEKLRDWDKDYNSVS--KELGKTGGRPRKKDSEEEPEKKPEEVTKKKRKYKLKRVIHNGFK--NETVEGFKNETVEGFKNETVTVSKIKPNNYS--------NIFNNLSNISTNVSNNLLIDDDEEIENEPTGRTINRSLLF-------SQEDIKQAYQFINR-FSVIQLRENFSFDKHFEERLVCYLWKAGISTFYTHEISKMIKKIADYEKSKKGRLNPIRDRALYM------VNGLVMNRASSQSEHATYKLNQYKKQKEQEKQQQEQQRSRVPFYNWLEEREEQTEGQLPTT 4CVH Chain:A ((1-411)) SMHPQAVAAVLPAGPTPKQ----FCPILERPLISYTLQALER---VCWIKDIVVAVTGENMEVMKSIIQKYQHKRISLVEAGVTRHRSIFNGLKALAE---------DQINSKLSKPEVVIIHDAVRPFVE--EGVLLKVVTAAKEHGAAGAIRPLVSTVVSPSADGCLDYSLERARHRASEMPQAFLFDVIYEAYQQCSDYDLEFGTECLQLALKYCCTKAKLVEGSPDLWKVTYKRDLYAAESIIKERISQEICVVMDTEEDNKHVGHLLEEVLKSELNHVKVTSEALDQCYNFVCVNVTTSDFQETQKLLSMLEESSLCILYPVVVVSVHFLDFKLVPP------SQKMENLMQIREFAKEVKERNILLYGLLISYP-----------------QDDQKLQESLRQGAIIIASLIKERNSGLIGQLLIA

General information: TITO was launched using: RESULT: Template: 4CVH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1783 88633 49.71 249.67 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 49.71 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.188

(partial model without unconserved sides chains): PDB file : Tito_4CVH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CVH-query.scw PDB file : Tito_Scwrl_4CVH.pdb: