Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFTAYQDARSWIHGRLKFGVKPGLGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNERISVNGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIMTACAFLYFAEFHKVDFVIFETGLGGRFDSTN---VVEPLLTVITSIGHDHMNILGNTIEEIAGEKAGIIKE---GIPIVTAVTQPEALQVIRHEAERHAAPFQSLHDACVIFNEEA---LPAGEQFSFKTEEKCYEDIRTSLIGTHQ---RQNAALSILAAEWLNKENIAHISDEALRSGLVK-AAWPGRLELVQEHPPVYLD--GAHNEEGVEKLAETMKQRFANSRISVVFSALK---D---KPYQNMIKRLETIAHAIHFASFDFPRASLAKDLYDASEISNKSWSEDPDDVIKFIESKKGSNEIVLITGSLYFISDIRKRLK 3ZL8 Chain:A ((81-410)) ---------------------------------------SGTVVGVTGSSGKTTTKEILYNLLKNKR---SVFKTPGN--MNTEY------------------------------GLPLSILN----------DYK-GEEILVLEMAASRPGDIAHLCKIAPPDVAVLLNVGSAHLEFFG-TRERIMETKMEIIKHSKENAIAVTLFDDPDLRKEVP-RY----RNTLFFGKEGGDSVLKDWWYYEGSTIAEFEA---FDSLFTVKLSGYWNGGQLLNIAASLCVMRTLG------ETVDI--FDLASLKTVPGRFNVREKKGVLIVDDTYNASPEAFQTSIEALLRF-P-GKKFAVVGAMKELGERSKEFHEELGERLNV-LDGVYVFLSEPE-AEWIKS--KKIILKSDDPEKIAKD----LATRVKKGDVVLFKASRAV---------

General information: TITO was launched using: RESULT: Template: 3ZL8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1751 985 0.56 3.19 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 0.56 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.351

(partial model without unconserved sides chains): PDB file : Tito_3ZL8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZL8-query.scw PDB file : Tito_Scwrl_3ZL8.pdb: