Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMPRTIIEKIWDQHIVKHGEGKPDLLYIDLHLIHEVTSPQAFEGLRQK---GRKV-RRPQNT------FATMDHNIPTVNR-------FEIK---DEVAKRQVTALERNC--EEFGVRLADLHSVDQGIVHVVGPE---------LGLTLPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLSTQTLWQQRPKTLEVRVDGTLQKGVTAKDVILAVIGKYGVKFGTGYVIEYTGEVFRNMTMDERMTVCNMSIEAGARAGLIAPDEVTFEYCKNRKYTP----KGEEFDKAVEEWK-ALRTDPGAVYDKSIVLDGNKISPMVTWGINPGMVLPVDSEVPAPESFSAED-DKKEA-------IRAYEYMGLTPHQKIEDIKVEHVFIGSCTNSRMTDLRQAADMIK------GKKVADSVRAIVVPGSQSVKLQAEKEGLDQIFLEAGFEWRESGCSMCLSMNNDVVPE--------GE---RCASTSNRNFEGRQG-KGARTHLVSPAMAAMAAIHGHFVDVRKFYQEKTVV 2B3Y Chain:A ((72-569)) --------------------NIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPEKINPVCPADLVIDHSIQVDFNRRADSLQKNQDLEFERNRERF-EFL-KWGSQAFHNMRIIPPG---SGIIHQVNLEYLARVVFDQDGYYYPD-SLVGTDSHTTMIDGLGILGWGVGGIEAEAVMLGQPISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHLRQVGVVGKFVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSITYLVQTGRDEEKLKYIKKYLQAVGMFRDFNDPSQDPDFTQVVELDLKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGFKGFQVAPEHHNDHKTFIYDNTEFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLNVMPYIKTSLSPGSGVVTYYLQESGVMPYLSQLGFDVVGYGCMTCIGNS-GPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTIRIDF---------

General information: TITO was launched using: RESULT: Template: 2B3Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2356 -10745 -4.56 -24.64 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -4.56 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.372

(partial model without unconserved sides chains): PDB file : Tito_2B3Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2B3Y-query.scw PDB file : Tito_Scwrl_2B3Y.pdb: