Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVYHFVGIKGTGMSPLAQILHDNGYTVQGSDIEKFIFTQTALEKRNITIL-PFSAENIKPGMTVIAGNAFPDTHPEIEKAMSEGIPVIRYHKFLGDYMK--KFTSVAVTGAHGKTSTTGLLAHVIQNAK--PTSFLIGDGTGQGNE-----NSEYFVFEACEYRRHFLSYQPDYAIMTNIDFDHPDYFS-SIDDVFDAFQEMALQVNKGIIACGDDEHLPKIHANVPVVYYGTGEENDFQARNIVKSTEGTTFDVFVRNTFYDTFYIPAYGHHNVLNSLAVIALCHYEEIDSSIIKHALKSFGGVKRRFNEKQ---LGDQVLIDDYAHHPTEIKVTIEAARQKYPDREIVAVFQPHTFTRTQQFLDEFAESLSGADCVYLCDIFGSARENAGKLTIGDLQGKIH----NAKLI--EEDDTSVLKAHDKAVLIFMGAGDIQKYMRAYENVMA 1J6U Chain:A ((0-442)) HMKIHFVGIGGIGMSAVALHEFSNGNDVYGSNIEET-ERTAYLRKLGIPIFVPHSADNWYDPDLVIKTPAVRDDNPEIVRARMERVPIENRLHYFRDTLKREKKEEFAVTGTDGKTTTTAMVAHVLKHLRKSP-TVFLGGIMDSLEHGNYEKGNGPVVYELDESEEFFSEFSPNYLIITNARGDHLENYGNSLTRYRSAFEKISRNTD-LVVTFAEDELTSHLG----DVTFGVKK-GTYTLEMRSASRAEQKAMVEKNGKRYLELKLKVPGFHNVLNALAVIALFDSLGYDLAPVLEALEEFRGVHRRFSIAFHDPETNIYVIDDYAHTPDEIRNLLQTAKEVFENEKIVVIFQPHR--------GNFAKALQLADEVVVTEVYD---------SGKMIWDSLKSLGKEAYFVEKLPELEKVISVSENTVFLFVGAGDIIYSSRRFVERY-

General information: TITO was launched using: RESULT: Template: 1J6U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2343 -47131 -20.12 -116.08 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -20.12 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.530

(partial model without unconserved sides chains): PDB file : Tito_1J6U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1J6U-query.scw PDB file : Tito_Scwrl_1J6U.pdb: