Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNHSKTLLLTAAAGLMLTCGAVSSQAKHKLSDPYHFTVNAAAETEPVDTAGDAADDPAIWLDPKTPQNSKLITTNKKSGLVVYSLDGKMLHSYNTGKLNNVDIR--YDFPLNGKKVDIAAASNRSEGKNTIEIYAIDGKNGTLQSMTDPDHPIATAINEVYGFTLYHSQKTGKYYAMVTGKEGEFEQYELKADKNGYISGKKVRAFKMNSQTEGMAADDEYGRLYIAEEDE-AIWKFSAEPDGGSNGTVIDRADGRHLTRDIEGLTIYYAADGKGYLMASSQGNSSYAIYDRQGKNKYVADFRITDGPETDGTSDTDGIDVLGFGLGPEYPFGIFVAQDGENIDHGQKANQNFKIVPWERIADQIGFRPLANEQVDPRKLTDRSGK 1Q7F Chain:A ((759-1037)) -IKRQRMIYHCKFG-------EFGVMEGQFTEPSGVAVN--------------------------AQNDIIVADTNNHRIQIFDKEGRFKFQFGECGKRDSQLLYPNRVAVVRNSGDIIVTERSPT--HQIQIYNQYGQFVRKFGATILQHPRGVTVDNKGRIIVVEC---KVMRVIIFDQNGNVLHKFGCSKHLEFPNGVVV---------------NDKQEIFISDNRAHCVKVFN------YEGQYLRQIGGEGITNYPIGVGI---NSNGEILIADNHNNFNLTIFTQDGQLISALESKVKHAQCFDVALMDDG--------------SVVLASK----------DYRLYIYRYVQLAP--------------------VGM

General information: TITO was launched using: RESULT: Template: 1Q7F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1709 41823 24.47 151.53 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 24.47 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.345

(partial model without unconserved sides chains): PDB file : Tito_1Q7F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Q7F-query.scw PDB file : Tito_Scwrl_1Q7F.pdb: