Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELYECIQDIFGGL--KNPSVKDLATSLKQIPNAAKLSQPYIKEPD--QYAYGRNAIYRN--NELEIIVINIPPNKETTVHDHGQSIGCAMVLEGKLLNSIYRSTGE--HAELSNSYFVHEGECLIST--KGLIHKMSNPTS-ERMVSLHVYSPPLEDM--TVFEEQKEVLENS 4QMA Chain:A ((17-182)) REFITGLSALLDEQPGEARILREGGALLARLV-ARDDWLPDAFAQPHPEY-YQQMLLHCDSAERFSIVSFVWGPGQRTPIHDHT-VWGLIGMLRGAEYSQPFVLDGSGRPVLHGEPTRLEPGHVEAVSPTVGDIHRVHNAYDDRVSISIHVYGANIGGVRRSVYTEAGE-----

General information: TITO was launched using: RESULT: Template: 4QMA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 738 9985 13.53 65.26 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 13.53 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_4QMA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QMA-query.scw PDB file : Tito_Scwrl_4QMA.pdb: