Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENFTYYNPTKLIFGKGQLEQLRKEFKRYGKN-VLLVYGGGSIKRNGLYDQVTGILKEEGAVVHELSGVEPNPRLATVEKGIGLCREHDIDFLLAVGGGSVIDCTKAIAAGVKYDGDAWDIF--SKKVT-AEDALPFGTVLTLAATGSEMNPDSVITNWETNEKFVWGSNVTHPRFSILDPENTFTVPENQTVYGMVDMMSHVFEQYFHNVENTPLQDRMCFAVLQTVIETAPKLLEDLENYELRETILYAGTIALNGTLQMG-YFGDWASHTMEHAVSAVYDIPHAGGLAILFPNWMRYTLDTNVGRFKNLMLNMFDIDTEGKTDKEIALEGIDKLSAFWTSLGAPSRLADYNIGEEKLELIADIAAKEMEHGGFGNFQKLNKDDVLAILRASL 5BR4 Chain:A ((1-383)) MMANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLV-QCGVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDTCKAIGIISNNPEF-ADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRRKFVCVDPHDIPQVAFIDADMMDGCPPALKAATGVDALTHAIEGYITRGAW-ALTDALHIKAIEIIAGALRGSVAG--DKDAGEEMALGQYVAGMGFSNVGLG----LVHGMAHPLGAFYNTPHGVANAILLPHVMRYNADFTGEKYRDIARV-MGVKVEGMSLEEARNAAVEAVFALNRDVGIPPHLRDVGVRKEDIPALAQAALDDVC--TGGNPREATLEDIVELYHTAW

General information: TITO was launched using: RESULT: Template: 5BR4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2406 -54676 -22.72 -144.65 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -22.72 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.551

(partial model without unconserved sides chains): PDB file : Tito_5BR4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BR4-query.scw PDB file : Tito_Scwrl_5BR4.pdb: