Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIAVVTDSTAYIPKEMREQHQIHMIPLQVVFREETYRE-E-IELDWKSFYEEVKKHNELPTTSQPPIGELVALYEELGKSYDAVISIHLSSGISGTFSSAAAADSMV----DNIDVYPFDSEISCLAQGFYALKAAELIKNGASSPEDIIKELEEMKKTVRAYFMVDDLAHLQRGGRLSSAQAFIGSLLKVKPILHFD-NKVIVPFEKIRTRKKAISRIYELLDEDASKGLPMRAAVIHANREEEAAKIIEELSAKYPHVEFYNSYFGAVIGTHLGEGALGICWCFK 3PL5 Chain:A ((36-317)) -TFKILTDSTADLPESWTQENDVQVLGLTVQLDGITYETVGPDRLTSRVLLEKIAA-GSKPTTSQVNVGQFESYFRQSAENGQEVLYIAFSSVLSGTYQSAVMARDIVLEEYPQASIEIVDTLAATGGEGYLAMLAAQAREEGKS-LKETKELILDVGPRLRTFFLVDNLYHLMRGGRLS--------LVNIKPLLWLDASGKLVPIAKLRGRKKGMKEMLKRATADV-A--HDTAVVAYANDSEAAENLKEQLLANEKIKNVVTLPLGPVISTHVGPNTLAVFTIGK

General information: TITO was launched using: RESULT: Template: 3PL5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1479 29492 19.94 110.46 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : 19.94 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.486

(partial model without unconserved sides chains): PDB file : Tito_3PL5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PL5-query.scw PDB file : Tito_Scwrl_3PL5.pdb: