Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSCKQLIVCSLAAILLLIPSVSFAADSNISVKLLNYIGNKSSISLSPTGFYKVTGDNVAVTDRFAGASRYETATLASNSQWKNPNTVILVNRDIFIDALPVIPLAKKLNAPVLFTQPDTLTKTTER-QIAKFNPDNILIIGGARSISKDVENKLKSYGAVKRISGKNRYVLSENIAKQMGSYDKAIVVTGRVFQDALA-IAPYAAAHGYPILLTEKDKLPDYD---LPKQVIIIGSSFSVSDSVENQIKK----TSTVQRIPGSTRYELTANIIKQLKLKADKVVMTNGTKYADVLIGASLASKKNSQILFVKQDSVPAAAKSITKDKATYAYDFIGSTSSISAEVENSLADEFYLADGGTYNLKINSGKLNLENIKTYGNSLRIKPENYSTSNRISLDGKQYLGTVNFSIESTKYIRPVNENIPF--EDYLKGVIPNEMPASWSLEALKAQTVAARTYSITKTGTTV---PDTTAFQVYGGYSWNSNTNKAVEQT-KGKVLKYNGSLITAAYSSSNGGYTEASNEVWSSSVPYLIAKKDTKDPQIGWTLTLSKQQLDTKSLDLTKPSSWWSSATETDSARLSGVKNWILKNKETSADSVKIASIDDLSFSGTTQGQRAKTASMKVKYFVKSSTGSYNLSKITTISVPTSELRTMIGATVFKSTYVTVKKDTSKYTISGKGYGHGIGMSQYGAKARAEAGDSYSSILKFYYPGTTLTSY- 5F9H Chain:A ((11-692)) FKPRNYQLELALPAMKG--KNTIICAPTGCGKTFVSLLICEHHLKKFPQGQ---KGKVVFFANQ---IPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVE--NNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGS----SGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAFDEIEQDLTQRF---EEKLQELESVSRDPSNEN--------------------------PKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLT-------------SNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQPKPVPDKENKKLLCRKCKALACYTADVRVIEECHY--TVLGDAFKECFVSRPHPKPKQFSS--FEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQT---------------LYSKWKDFHFEKIPFDPAE

General information: TITO was launched using: RESULT: Template: 5F9H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3100 6175 1.99 9.80 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 1.99 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.122

(partial model without unconserved sides chains): PDB file : Tito_5F9H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F9H-query.scw PDB file : Tito_Scwrl_5F9H.pdb: