Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVFDVHKFDMKKEQDFLQVQFNLKNRINLSPTIH------PDSINTCAGVDLAYWEQDGEPYGVCCIIVIDADTKEVIEKVHSMGRISVPYVSGFLAFRELPLIIEAAKKLET-----EPDVFLFDGNGYLHYNHMGVATHAAFFLGKPTIGIAKTYLKIKGCDFVT-------PEIEVGAYTDI-IIDGEVYGRALRTR-RDVKPIFLSCGNYIDLDSSYQITMSLINQESRLPIPVRLADLETHVLRTFYQKNHV 4NSP Chain:A ((13-252)) ------------TLSLWKREQARLKAHVVDRDTEAWQRDPAFSGLQRVGGVDVSFV--DS-VRACASLVVLSFPELEVVYEESRMVSLTAPYVSGFLAFREVPFLLELVQQLREKEPGLMPQVLLVDGNGVLHHRGFGVACHLGVLTDLPCVGVAKKLLQVDGLENNALHKEKIRLLQTRGDSFPLLGDSGTVLGMALRSHDRSTRPLYISVGHRMSLEAAVRLTCCCC--RFRIPEPVRQADICSREHIRKSAA---

General information: TITO was launched using: RESULT: Template: 4NSP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1182 -36159 -30.59 -165.87 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -30.59 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.422

(partial model without unconserved sides chains): PDB file : Tito_4NSP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4NSP-query.scw PDB file : Tito_Scwrl_4NSP.pdb: