Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MERKTVLVIADLGGCPPHMFYKSAAEKYNLVSFIPRPFAITASHAALIEKYSVAVIKDKDYFKSLADFEHPDSIYWAHEDHDKPEEEVVEEIVKVAGMFAVDAITTNNELFIAPMAKACERLGLRGAGVQAAENARDKNKMRAAFNRAGVKSIKNKRVTTLEDFRAALQEIGTPLILKPTYLASSIGVTLIKEMETAEAEFNRVNEYLKSINVPKAVTFEAPFIAEEFLQGEYDDWYETSGYSDYISIEGIMADGEYFPVAIHDKTPQ--IGFTETSHITPSILDDDAKRKIVEAAKKANEGLGLENCATHTEIKLMKNREAGLIESAARFAGWNMIP-NIKKVFGVDMAQLLLDVLCFGKEADLPKGLLEQEPCYVADCHLYPQHFKENGQLPETAVDFVIESIDIPDGVLKGDTEIVSFSAAEAGTSVDLRL-FEAFNSIAAFELKGSNSGDVAESIKQIQQQAKLTAKYALPV 3VOT Chain:A ((3-417)) KRNKNLAIICQ-N-KHLPFIFEEAERL-GLK-----VTFFYNSAEDFPGNL-----------------PAVERCVPLPLF--EDEEAAMDVVRQTFVEFPFDGVMTLFEPALPFTAKAAEALNLPGLPFTTMENCRNKNKTRSILQQNGLNTPVFHEFHTLADLEN--RKLSYPLVVKPVN-----GVVRVDDRKELEEAVRKVEAVNQRD-LNRFVHGKTGIVAEQFIDGPE------------FAIETLSIQGNVHVLSIGYKGNSKGPFFEEGVYIAPAQLKEETRLAIVKEVTGAVSALGIHQGPAHTELRLDKDGTPYVIEVGARIGGSGVSHYIVKESTGINFMQLVLQNALKPLESSEFEGE-IR---------PVRTAGNYIIP-VQGSGTFEKI--DGLEEVKQRQEVKRVFQFMRRGAKILPYPHFSGYPGFILTS--HHSYEECEAFYRELDDELHIIYQN----

General information: TITO was launched using: RESULT: Template: 3VOT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2211 -74058 -33.50 -182.41 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -33.50 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.467

(partial model without unconserved sides chains): PDB file : Tito_3VOT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3VOT-query.scw PDB file : Tito_Scwrl_3VOT.pdb: