Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREELKGIFASVFGEKEGLRFFFAPGRVNLIGEHTDYNGGHVFPCALTMGTYAAVAERNDG--LVRMYSDNFRNA-GIKECSLDD--I-RYQKEDDWANYPKGVIY----EFQQR-----GYAVPHGFDIVFSGNIPNGAGLSSSASIELLMGVVLQSYF--HPEVDALELVKMAQHAENHFIGVNCGIMDQFAIGMGKKHHAMLLNCDTL-DYEYSKLNV---SGLALVIANTNKKRTLA---DSSYNTRRQECNDALLDLKKGLD-------------IAALGDIKPSDF------------------DAHS--------------------------SLI----------------QNETNRRRAKHAVYENHRAIKTAHMFKEN-------NIDEIGQLMKESHLSLKDDYEVTCPELDELVFAAWDHEGVIGSRMTGAGFGGCTISIVKDEF---VDDFIQKVGDRYQEKTG--------LRADFYVADIGEGARELKGE 3V2U Chain:C ((24-519)) --LAVVDAFFQTYHV-KPDFIARSPGRVNLIGEHIDYCDFSVLPLAIDVDMLCAVKILDEKNPSITLTNADPKFAQRKFDLPLDGSYMAIDPSVSEWSNYFKCGLHVAHSYLKKIAPERFNNTPLVGAQIFCQSDIPTGGGLSSAFTCAAALAT-IRANMGKNFDISKKDLTRITAVAEHY-VGVNNGGMDQATSVYGEEDHALYVEFRPKLKATPFKFPQLKNHEISFVIANTLVKSNKFETAPTNYNLRVIEVTVAANALATRYSVALPSHKDNSNSERGNLRDFMDAYYARYENQAQPWNGDIGTGIERLLKMLQLVEESFSRKKSGFTVHEASTALNCSREEFTRDYLTTFPVRFQVLKLYQRAKHVYSESLRVLKALKMMTSATFHTDEDFFTDFGRLMNESQASCDKLYECSCIETNQICSIALA-NGSFGSRLTGAGWGGCTIHLVPSGANGNVEQVRKALIEKFYNV-RYPDLTDEELKDAIIVSKPALGTCLYE--

General information: TITO was launched using: RESULT: Template: 3V2U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2000 -7022 -3.51 -18.43 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain C : 0.69 3D Compatibility (PKB) : -3.51 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_3V2U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3V2U-query.scw PDB file : Tito_Scwrl_3V2U.pdb: