Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLSELKTSGHPLTLLCSFLYFDVSFMIWVMLGALGVYISQDFG--------------LSPFEKGLVVAVPILSGSVFRIILGILTDRIGPKKTAVIGMLVTMIPLLWGTF---GGRSLTELYAIGILLGVAGAS-FAVALPMASRWYPPHLQGLAMGIAG-AGNSGTLFATLFGPRLAE--QFGWHIVMGIALIPLLIVFILFVSMAKDSPAQPS---P--Q--------------------------------PLKSYLHVFGQKETWFFCLLYSVTFGG-----FVGLSSFLSIFFVDQYQLSKIHAGDFVTLCVAAGSFFRPVGGLISDRVGGTKVLSVLFVIVALCMAGVSSLP---------SLSMVIVLLFVGMMGLGMGNGAVFQLVPQRFRK-EIGMVTGIVGAAGGIGGFFLPNILGSLKQMTGTYAIGFITFSCIALLAFALVLAAGYYWRKSWSAESSPADV 4ZW9 Chain:A ((47-453)) -------------------------GYNTGVINAPEKIIKEFITKTLTDKGNAPPSEVLLTSLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLIVNLLAVTGGCFMGLCKVA-KSVEMLILGRLVIGLFCGLCTGFVPMYIGEISPTALRGAFGTLNQLGIVVGILVAQIFGLEFILGSEELWPLLLGFTILPAILQSAA-LPFCPESPRFLLINRKEEENAKQILQRLWGTQDVSQDIQEMKDESARMSQEKQVTVLELFRVSSYRQPIIISIVLQLSQQLSGINAVFYYSTGIFKD---AGVQEPIYATIGAGVVNTIFTVVSLFLVERAGRRTLHMIGLGGMAFCSTLMTVSLLLKDNYNGMSFVCIGAILVFVAFFEIGPGPIPWFIVAELFSQGPRPAAMAVAGCSNWTSNFLVGLLFPSAAHYLG-AYV------------------------------------

General information: TITO was launched using: RESULT: Template: 4ZW9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1590 -247992 -155.97 -742.49 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -155.97 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.272

(partial model without unconserved sides chains): PDB file : Tito_4ZW9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZW9-query.scw PDB file : Tito_Scwrl_4ZW9.pdb: