Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGFSHFNEQGRAQMVDISEKSSTVRTAAAVSSVHMKNEVYEKIQSHDIGKGDVLAVAQVAGIMAAKQTSNIIPMCHPLSLSGVDISFDWKIKEAEVILHIKAQVKTKGSTGVEMEALTSASVCALTVYDMCKALDKGMVIGPTFLLEKTGGKNGDFKRELSEYNLEDQK 2IDE Chain:K ((9-156)) ----------GRPRMVDVTEKPETFRTATAEAFVELTEEALSALEKGGVGKGDPLVVAQLAGILAAKKTADLIPLCHPLPLTGVEVRVE--LLKAEKRVRIEATVKTKAETGVEMEAMTACAVAALTVYDMLKAASKGLVISQVRLLHKAGGKSGEWRRE----------

General information: TITO was launched using: RESULT: Template: 2IDE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain K - contact count / total energy / energy per contact / energy per residue : 747 -74249 -99.40 -501.68 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain K : 0.86 3D Compatibility (PKB) : -99.40 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_2IDE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IDE-query.scw PDB file : Tito_Scwrl_2IDE.pdb: