Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASNRYHSINEIMESQLFY-QITTPPKKTQKAQYDPEVEKFLEIIEFVKE-NGREPQKVPTDLTERSL-ASR-LIGIRKDPDRMEYLKEYDEIGLLEVRSKELT-----IPKISSIDDILKSGLSALLGDNL-NNDITRS-IFDTSSLQKVTTMPEYVAKRKKIKDFGKFEELFKKCHK-EITEGKRKILTFKNEQDIQSN-SFYILKGVLLYVEDVGERKKAKGKTNARLRCIFENGTESDMLLRSLSAELYKHGRRVTDNEDTLLDNVREDDVSTGFIYVLKSLSTDPQISSIKNLYKIGFT-TGSVENRI-RNAENQSTYLYAPVEIVTTYQVFNM-NASKFETAIHHALANNNLDVSILGANGKMLVPKEWFVVTLEDLQAVIDEIVMMVHLYD 3PFN Chain:A ((6-361)) ---TTFGPKACVLQNPQTIMHIQDPASQRLTWNKSPKS------VLVIKKMRDASLLQPFKELCTH-LMEENMIVYVEKKVLEDPAIASDESFGAVKKKFCTFREDYDDISNQIDFIICLGGDGTLLYASSLFQGSVPPVMAFHLGSLGFLTPFS-----------FENFQSQVTQVIEGNAAVVLRSRLKVRVVKQ---AMQYQVLNEVVID-----------------------RGPSSYLS----NVDVYLDGHLITTVQ------------GDGVIVSTPTGSTAYAAAA-------GASMIHPNVPAIMITPICPHSLSFRPI-VVPAGVELKIMLSPEARNTAWVSFDGRKRQEIRHGDSISITTSCYPLPSICVRDPVSDWFESLAQCLHH

General information: TITO was launched using: RESULT: Template: 3PFN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1565 -116023 -74.14 -373.06 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -74.14 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.238

(partial model without unconserved sides chains): PDB file : Tito_3PFN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PFN-query.scw PDB file : Tito_Scwrl_3PFN.pdb: