Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIADQTFVKKVNQKLLLKEILKNSPISRAKLSEMTGLNKSTVSSQVNTLMKENLVFEIGQGQSSGGRRPVMLVFNKKAGYSIGIDVGVDYISGILTDLEGTIILDQHHHLES-NSP-EITKDILIDMIHHFITRMPQSPYGLIGIGICVPGLIDKN-QKIVFTPNSNWRDIDLKSFIQEKFNVPVFIENEANAGAYGEKVFGAAKNHNNIIYASISTGIGIGVIINNHLYRGVSGFSGEMGHMTIDFNGPKCSCGNRGCWELYASEKALLKSLQTKEKKVSYQDIIDLAHLNDIGTLNALQNFGFYLGIGLTNILNTFNPQAIILRNSIIESHPMVLNSIRSEVSSRVYPQLGNSYELLPSSLGKNAPALGMSSIVIEHFLDIVKM 2HOE Chain:A ((22-376)) -------------ISRILKRIM-KSPVSRVELAEELGLTKTTVGEIAKIFLEKGIVVEEKDSP----RPTKSLKISPNCAYVLGIEVTRDEIAACLIDASMNILAHEAHPLPSQSDREET-LNVMYRIIDRAKDMMEKLGSKLSALTVAAPGPIDTERGIIIDPRNFPLSQIPLANLLKEKYGIEVWVENDADMGAVGEKWYT--KRDDSFAWILTGKGIGAGIIIDGELYRGENGYAGEIGYTRVFNG------NEYVFLEDVCNENVVLKHVLS----MGFS-LAEARDSGDVRVKEYFDDIARYFSIGLLNLIHLFGISKIVIGGFFKELGENFLKKIKIEVETHLLY--KHSVDMSFSKVQEPVIAFGAAVHALENYLER---

General information: TITO was launched using: RESULT: Template: 2HOE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1827 -253027 -138.49 -729.18 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -138.49 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.471

(partial model without unconserved sides chains): PDB file : Tito_2HOE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HOE-query.scw PDB file : Tito_Scwrl_2HOE.pdb: