Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANVLMIGFPGEGHINPSIGVMKELKSRGENITYY--AVKEYKEKITA------LDIEFREYHDFRGDYFGKNATGDEERDFTEMLCAFLKACKDIATH-IYEEV--KHESYDYVIYDHHLLAGKVIANMLKLPRFSLCTTFAMNE---EFAKEMMG-AYMKG-SLE----DSPHYESYQ--QLAE-TLNA--DFQAEIKKPFDVFLADGDLTIVFTSRGFQPLAEQ----FGERYVFVGPSI-T-ER----A---GNNDFPFDQIDNENVLFISMGTIFNNQKQFFNQCLEVCKDFDGKVVLSIGKHIKT-S--E-LNDIPENFIVRPYVPQLEILKRAS--LFVTHGGMNSTSEGLYFETPLVVIPMGGDQFVVADQVEK-VGAGKVIKKEELSESLLKETIQEVMNNR---SYAEKAKEIGQSLKAAGGSKKAADSILEAVKQKTQSANA 3HBF Chain:A ((13-434)) LLHVAVLAFPFGTHAAPLLSLVKKIATEAPKVTFSFFCTTTTNDTLFSRSNEFLPNIKYYNVHDGLPKGYVSS-GNP---R--EPIFLFIKAMQENFKHVIDEAVAETGKNITCLVTDAFFWFGADLAEEMHAKWVPLWTAGPHSLLTHVYTDLIREKTGSKEVHDVKSIDVLPGFPELKASDLPEGVIKDIDVPFATMLHKMGLELPRANAVAINSFATIHPLIENELNSKFKLLLNVGPFNLTTPQRKVSDEHGCLEWLD--QHENSSVVYISFGSVVTPPPHELTALAESLEECGFPFIWSFRGDPKEKLPKGFLERTKTKGKIVAWAPQVEILKHSSVGVFLTHSGWNSVLECIVGGVPMISRPFFGDQGLNTILTESVLEIGVGVDNGVLTKESIKKALELTMSSEKGGIMRQKIVKLKESAFKA-----------------------

General information: TITO was launched using: RESULT: Template: 3HBF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1998 2268 1.14 6.06 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 1.14 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.525

(partial model without unconserved sides chains): PDB file : Tito_3HBF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HBF-query.scw PDB file : Tito_Scwrl_3HBF.pdb: