Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKLDEMTAYLPPFLTKLKEMAELLKAEAPEFEKQNNSIFDLTDQLFVTTATWGLERWEKILNVPRESGDTDEIRRLRLISKMSNIPPATYRAIEHALNRFLKNPSAQVRLLPGEYRFKVDIDIDDMQHMSELIETLENMKPAHLAYTLRAALNEPLQIKDTVILNNRRYRKASELKVGYSVTLNNNEVVLV 3ZMD Chain:A ((27-174)) TRWLT--DTEQCAWRTHLEVNRLLTHQLEKDLQPFGLT-----------------MNDYEILVNLSESEG----DRMR-MSDLATATMQSKSRLSHQITRMENANLVRRENCESDRRGL------FAVLTEHGLETMRKVAPHHVA-SVRRHFIDLLAPEDLTELDKALKPIAEHLRGQ-------------

General information: TITO was launched using: RESULT: Template: 3ZMD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 409 -26469 -64.72 -178.84 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -64.72 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.393

(partial model without unconserved sides chains): PDB file : Tito_3ZMD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZMD-query.scw PDB file : Tito_Scwrl_3ZMD.pdb: