Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTVKVNIVTPDGPVYDADIEMVSVRAESGDLGILPGHIPTVAPLKIGAVRLKKDGQTEMVAVSGGFVEVRPDHVTILAQAAETAEGIDKERAEAARQRAQERLNSQSDDTDIRRAELALQRALNRLDVAGK 2E5Y Chain:A ((1-131)) MKTIHVSVVTPDGPVYEDDVEMVSVKAKSGELGILPGHIPLVAPLEISAARLKKGGKTQYIAVSGGFLEVRPDKVTILAQAAERAEDIDVLRAKAAKERAERRLQSQQDDIDFKRAELALKRAMNRLSVAE-

General information: TITO was launched using: RESULT: Template: 2E5Y.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 579 -67212 -116.08 -513.06 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.94 3D Compatibility (PKB) : -116.08 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.609

(partial model without unconserved sides chains): PDB file : Tito_2E5Y.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2E5Y-query.scw PDB file : Tito_Scwrl_2E5Y.pdb: