Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKNILLVGMLVLLLMFVSACSGTASKGSSSDSASEKTEMRTYKSPKG--NVNIPAHPKRIVTDF--YAGELLSVGANVVGSGSWSFDNPFLKSKLKNVKDVGD--PISVEKVMELQPDLIVVMNE-ENVDKLKKIAPTVVIPYNTAKNVEDTVSMFGDIAGAKDQAKSFMADFNKKAEAAKKKIAGVIDKDATFGIYENTDKGEFWVFNDNGGRGGQAVYNALGLKAPEKIEQDVIKKGEMKQLSQEVIPEYAADYMFITDYNPKGESKTLDKLENSSIWKNLDAVKHNRVFINDFDSFYPYDPISVSKQVDIITDMLIKRAEENKK 5FLY Chain:A ((3-280)) -----------------------------------KETKAFNLKTAKGEEKIDIPKDPKRIVVMAPTYAGGLKYLDANIVGVSDQVDQSPVLA---KQFKDVDKVGAEDVEKVASLKPDLIITYNTDKNTDKLKKIAPTIAFDYA-KYNYLEQQEAMGDIVGKSDEVKKWKADWEKQTAQDSKDIKAHLGDDTSVTIFEDFD-KKIYAYGKNWGRGSE-VLYQAFGLQMPKALDDATKKEGWTEVPKEEVGKYAGDVIITAKAKD----AAQPEFQKTAMWQNLEAVQNKYAFNVDSSVYWYNDPYTLDVIRKDLKKQLLALP-----

General information: TITO was launched using: RESULT: Template: 5FLY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1436 90878 63.29 335.34 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 63.29 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.565

(partial model without unconserved sides chains): PDB file : Tito_5FLY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FLY-query.scw PDB file : Tito_Scwrl_5FLY.pdb: