Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLPAKTFVILCILFLLDLCFSYIRHEWHSQNALQDM---PVPSDLHPIVKQNADALKAAAANKGIDVVITEGFRSFKEQDELYKQGRTKKGN---IVTYARGGESYHNYGLAIDFALQKKDGSIIWDMEYDGNQNGKSDWLEVVEIAKTLGF--EWGGDWKR----FKDYPHLEMIPN 4MUQ Chain:A ((17-173)) ------------NLLLVNKNYPLRD---NNVKGLVPADIRFPNILMKRDVANVLQLIFEKI-SAGNSIVPVSGYRSLEEQTAIYDGSLKDNGEDFTRKYVALPNHSEHQTGLAIDLGLNKKDIDFI-R--PDFPYDGICDEF--RRAAPDYGFTQRYARDKEEITGISHEPWHFRYVG-

General information: TITO was launched using: RESULT: Template: 4MUQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 613 -52782 -86.10 -364.01 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -86.10 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.310

(partial model without unconserved sides chains): PDB file : Tito_4MUQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MUQ-query.scw PDB file : Tito_Scwrl_4MUQ.pdb: