Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHVITTQVLFIFCFLLLIHSIETLAYATRLSGARVGFIASALSLFNVMVIVSRMSNMVQQPFTGHLIDDAGKNALAIVGEQFRFLIFGSTVGTILGIILLPSFVALFSRAIIHLA-GGGGSVFQVFRKGFSKQGFKNALSYL--RLPSISYVKGFHMRLIPKRLFVINMLITSIYTIGVLSALYAGLLAPERSTTAVMASGLINGIATMLLAIFVDPKVSVLADDVAKGKRSYIYLKWTSVTMVTSRVAGTLLAQLMFIPGAYYIAWLTKWF 1M4I Chain:A ((1-181)) MHT-------------QVHTARLVH-------------TADLDSETRQDIRQMVTGAFAGDFTETDWEHTLGGMHALIWHHGAIIAHAAVIQRRLIYRGNALRCGYVEGVAVRADWRGQRLVSALLDAVEQVMRGAYQLGALSSSARARRLYASRGWLPWHGPTSVLA---------------------PTGPVRTPDDDG---TVFVLPIDISLDTSAELMCD---------------------------------WRAG-------DVW-

General information: TITO was launched using: RESULT: Template: 1M4I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 840 -128332 -152.78 -720.96 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain A : 0.52 3D Compatibility (PKB) : -152.78 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.184

(partial model without unconserved sides chains): PDB file : Tito_1M4I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1M4I-query.scw PDB file : Tito_Scwrl_1M4I.pdb: