Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFWKNKNVFVTGCTGLLGSYLVKELIEQGANVTGLVRDHVPQSNLYQGEHIKKMNIVRGSLEDLAVIERALGEYEIDTVFHLAAQAIVGVANRNPISTFEANILGTWNILEACRKHPLIKRVIVASSDKAYGDQENLPYDENMPLQGKHPYDVSKSCADLISHTYFHTYGLPVCITRCGNLYGGGDLNFNRIIPQTIQLVLNG-EAPEIRSDGTFVRDYFYIEDAVQAYLLLAEKMEENNLAGEAFNFSNEIQLTVLELVEK-ILKKMNSNLKPKVLNQGSNEIKHQYLSAEKARKLLNWTPAYTIDEGLEKTIEWYTEFFKK 3KO8 Chain:A ((1-311)) ------MRIVVTGGAGFIGSHLVDKLVELGYEVVVVDNLSSG--RREFV--NPSAELHVRDLKDYSW-GA---GIKGDVVFHFAANPEVRLSTTEPIVHFNENVVATFNVLEWARQT-GVRTVVFASSSTVYGDADVIPTPEEEPYKPISVYGAAKAAGEVMCATYARLFGVRCLAVRYANVVGPRLRH--GVIYDFIMKLRRNPNVLEVL----QRKSYLYVRDAVEATLAAWKKFEEMDAPFLALNVGNVDAVRVLDIAQIVAE-VLGLRPEIRLV----GDVKYMTLAVTKLMKLTGWRPTMTSAEAVKKTAEDLAKELW-

General information: TITO was launched using: RESULT: Template: 3KO8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1801 -198246 -110.08 -672.02 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -110.08 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_3KO8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3KO8-query.scw PDB file : Tito_Scwrl_3KO8.pdb: