Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHQVRCAVLGLGRLGYYHAKNLVTSVPGAKLVCVGDPLKGRAEQVARELGIEKWSEDPYEVLEDPGIDAVIIVTPTSTHGDMIIKAAENGKQIFVEKPLTLSLEESKAASEKVKETGVICQVGFMRRFDPAYADAKRRIDAGEIGKPIYYKGFTRDQGAPPA-EFIKHSGGIFIDCSIHDYDIARYLLGAEITSVSGHGRILNNPFMEQYGDVDQALTYIEFDSGAAGDVEASRTSPYGHD-IRAEVIGTEGSI-FIGTLRHQHVTILSAKGSSFDII-PDFQTRFHEAYCLELQHFAECVRNGKTPI-VTDIDATINLEVGIAATNSFRNGMPVQLDVKRAYTGM 3EZY Chain:A ((2-335)) ---SLRIGVIGLGRIGTIHAENLKM-IDDAILYAISDVREDRLREMKEKLGVEKAYKDPHELIEDPNVDAVLVCSSTNTHSELVIACAKAKKHVFCEKPLSLNLADVDRMIEETKKADVILFTGFNRRFDRNFKKLKEAVENGTIGKPHVLRITSRDPA-PPPLDYIRVSGGIFLDMTIHDFDMARYIMGEEVEEVFADGSVLVDEEIGKAGDVDTAVVVLRFKSGALGVIDNSRRAVYG-YDQRIEVFGSKGRIFADNVRET-TVVLTDEQGDRGSRYLYFFLERYRDSYLEELKTFIKNVKSGE-PPAVSGEDGKMALLLGYAAKKSLEEKRSVKLEEVI-----

General information: TITO was launched using: RESULT: Template: 3EZY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1787 -176730 -98.90 -537.17 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -98.90 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_3EZY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EZY-query.scw PDB file : Tito_Scwrl_3EZY.pdb: