Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKYGIVLFPSK------KLQDLANSYRK-RYDPSYSLIPPHLTLRASFECAEEKADQLVSHLRNIAKESHPLVLKMTKYSSF-APVNNVIYIKAEPTEELKTLNEKLYTGVLAGEQEYNFVPHVTVGQNLSDDEHSDVLGQLKMQEVSHE-EIVDRFHLLYQLENGSWTVYETFLLGRGE 4Z5V Chain:A ((7-167)) PNHFINFPLAQFSGFMGKYLKLQSQLVEMGLDCKLQK-APHVSI-TLLDIKADQYKQVEFAIQEIIDDLAAYEGDIVFDNPHMLGRCLVLDVR-GFEELHEDIVEILRRRGCTADQSRHWIPHCTVAQFDEERETK-------------GMQFYHKEPFYLKHNNLLTDAGLELVKI---

General information: TITO was launched using: RESULT: Template: 4Z5V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 623 -72996 -117.17 -480.23 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -117.17 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.369

(partial model without unconserved sides chains): PDB file : Tito_4Z5V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z5V-query.scw PDB file : Tito_Scwrl_4Z5V.pdb: