Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLYMSVDMEGISGLPDDTFVDSGKRNYERGRLIMTEEANYCIAEAFNSGCTEVLVNDSHSKMNNLMVEKLHPEADLISGDVKPFSMVEGLDDTFRGALF-LGYHARAST-PGVMSHSMIFGVRHFYINDRPVGELGLNAYVAGYYDVPVLMVAG--DDRAAKEAEELIPNVTTAAVKQTISRSAVKCLSPAKAGRLLTEKTAFALQNKDKVKPLTPPDRPVLSIEFANYGQAEWANLMPGTEIKTGTTTVQFQAKDMLEAYQAMLVMTELAMRTSFC 2Q28 Chain:A ((12-156)) ---------------------------------------HIIVEALKQNNIDTIYGVVGIPV--TDMARHAQA--------E-GIRYIGFRHEQ-SAGYAAAASGFLTQKPGICLTVSAPG-FL----N------GLTALANATVNGFPMIMISGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVS-----------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2Q28.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 666 -69073 -103.71 -489.88 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -103.71 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.320

(partial model without unconserved sides chains): PDB file : Tito_2Q28.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Q28-query.scw PDB file : Tito_Scwrl_2Q28.pdb: