Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTRKPFIICDFDGT-ITMND-NIINIMKTFAPP-------------EWMALKDGVLSKTLSIKEGVGRMFGLLPSSLKEEITSFVLEDAKIREGFREFVAFINEHE-IPFYVISGGMDFFVYPLLEGIVEKDRIYCNHASFDNDYIHIDWPHSCKGTCSN-QCGCCKPSVIHELSEPNQYIIMIGDSVTDVEAAK---LSDLCFARDYLL-NECREQNLNHLPYQDFYEIRKEIENVKEVQEWLQNKNAGESSLK 2HCF Chain:A ((3-230)) ---SRTLVLFDIDGTLLKVESMNRRVLADALIEVYGTEGSTDFSGKMDGAIIYEVLSNVGLERAEIADKFDKAKETYIALFRERARREDITLLEGVRELLDALSSRSDVLLGLLTGNFEASGRHKLKLP-GIDHYFPFGAFADDALDRNELPHIALERARRMT----------GANYSPSQIVIIGDTEHDIRCARELDARSIAVATGNFTMEELARHKPG-----------TLFKNFAETDEVLASILT------

General information: TITO was launched using: RESULT: Template: 2HCF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 982 -104030 -105.94 -509.95 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -105.94 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.323

(partial model without unconserved sides chains): PDB file : Tito_2HCF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HCF-query.scw PDB file : Tito_Scwrl_2HCF.pdb: