Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLAPHGGTLVNRVDESYDV----------SGIQKEIELDLISFADLELIGIGAYSPIEGFFNEKDYVSVVENMRLSSGVVWSLPITLPVDAQKAAELSLGETVKLTY-EGETYGVIQIEDLYVPDKQKEAVNVYKTDEQEHPGVKKLFS-RGNTYVGGPITLIKKAS-KQFPEFTFEPSETRRQFAEKGWETIVGFQTRNPVHRAHEYIQKTALET-VDGLFLNPLVGETKSDDIPADVRMESYQVLLDHYYPKDRVFLGVFLAAMRYAGPREAIFHALVRKNYGCTHFIVGRDHAGVG------DYYGTYEAQELFDTFKPEELGITPLKFEHSFFCKKCGNMGTAKTCPHGREHHVILSGTKVRGMLRDGVLPPAEFSRKEVVEVLIKGMKKKEEVGVS 1G8F Chain:A ((2-396)) --PAPHGGILQDLIARDALKKNELLSEAQSSDI-LVWNLTPRQLCDIELILNGGFSPLTGFLNENDYSSVVTDSRLADGTLWTIPITLDVDEAFANQIKPDTRIALFQDDEIPIAILTVQDVYKPNKTIEAERVFRGD-PEHPAISYLFNVAGDYYVGGSLEAIQLPQHYDYPGLRKTPAQLRLEFQSRQWDRVVAFQTRNPMHRAHRELTVRAAREANAKVLIHPVVGLTKPGDIDHHTRVRVYQEIIKRY-PNGIAFLSLLPLAMRMSGDREAVWHAIIRKNYGASHFIVGRDHAGPGKNSKGVDFYGPYDAQELVESYK-HELDIEVVPFRMVTYLPDEDRYAPIDQIDTTKTRTLNISGTELRRRLRVGGEIPEWFSYPEVVKILRESNPPRPKQGF-

General information: TITO was launched using: RESULT: Template: 1G8F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2004 -181198 -90.42 -483.19 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -90.42 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_1G8F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1G8F-query.scw PDB file : Tito_Scwrl_1G8F.pdb: