Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGKVYIVGAGPGDPDLLTIKALKAIEKADVILYDRLVNKEILQYAKEQADLIYCGKLPDFHTMKQETINRFLVKYAQKGKMVVRLKGGDPFVFGRGGEEAECLSENGIPFEIIPGITSGIAAAAYAGIPVTHRDAGSNVAFVT-----GHYKKEEDFEEKWKALATGIDTLVIYMGIKNVQQIERKLLENGRDGSTPAAFIHWGTTDKQKSVFCTVDTLSETVIKENITNPSLIVIGNVVNYHYKLEWFESELKKQDLSEAL 1V9A Chain:B ((1-223)) -GRVYLVGAGPGDPELLTLKAYRLLKEAPVVLYDRLVDERVLALA--PGEKVYVGK----------EIHRLLLRHARAHPFVVRLKGGDPMVFGRGGEEVLFLLRHGVPVEVVPGVTSLLA----SGLPLTHRGLAHGFAAVSGVLEGGGYPDLRPFAR--------VPTLVVLMGVGRRVWIAKELLRLGRDPREPTLFVERASTPKERRVHARLEEVAEG--KVEVRPPALWILGEVVR---------------------

General information: TITO was launched using: RESULT: Template: 1V9A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1133 -143948 -127.05 -688.74 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -127.05 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.591

(partial model without unconserved sides chains): PDB file : Tito_1V9A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V9A-query.scw PDB file : Tito_Scwrl_1V9A.pdb: