Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTMQIKIKYLDETQTRISKIEQGDWIDLRAAEDVTIKKDEFKLVPLGVAMELPEGYEAHVVPRSSTYKNFGVIQTNSMGVIDESYKGDNDFWFFPAYALRDTEIKKGDRICQFRIMKKMPAVELVEVEHLGNEDRGGLGSTGTK 2XCE Chain:B ((2-130)) -TMQIKIKYLDETQTRISKIEQGDWIDLRAAEDVTIKKDEFKLVPLGVAMELPEGYEAHVVPRSSTYKNFGVIQTNSMGVIDESYKGDNDFWFFPAYALRDTEIKKGDRICQFRIMKKMPAVELVEVEHL--------------

General information: TITO was launched using: RESULT: Template: 2XCE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 534 -68288 -127.88 -529.36 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.96 3D Compatibility (PKB) : -127.88 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.96 QMean score : 0.567

(partial model without unconserved sides chains): PDB file : Tito_2XCE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2XCE-query.scw PDB file : Tito_Scwrl_2XCE.pdb: