Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKNHFTFQKLNGITPYIWTIFFILPFYFIWKSSSTFVIIVGIILTLLFFSVYRFAFVSKGWTIYLWGFLLIGISTASITLFSYIYFAFFIAYFIGNIKERVPFHILYYVHLISAAVAANFSLVLKKEFFLTQIPFVVITLISAILLPFSIKSRKERERLEEKLEDANERIAELVKLEERQRIARDLHDTLGQKLSLIGLKSDLARKLIYKD----PEQAARELKSVQQTARTSLNEVRKIVSSMK-------GIRLKDELINIKQI----LEAADIMFIYEEEKWPENISLLNENILSMCLKEAVTNVVKHSQAK-TCRVDIQ---QLWKEVVITVSDDGTFKGEEN------------SFSKGHGLLGMRERLEFANGSLHI-DT-ENGTKLTMAIPNNSK 4GCZ Chain:A ((148-373)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RLSAMGEMASALAHELNQPLAAISNYMKGSRRLLAGSSDPNTPKVESALDRAAEQALRAGQIIRRLRDFVARGESEKRVESLSKLIEEAGALGLAGAREQNVQLRFSLDPG-ADLVLADRVQIQQVLVNLFRNALEAMAQSQRRELVVTNTPAADDMIEVEVSDTGSGFQDDVIPNLFQTFFTTKDTGMGVGLSISRSIIEAHGGRMWAESNASGGATFRFTLPAAD-

General information: TITO was launched using: RESULT: Template: 4GCZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 554 20891 37.71 108.24 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain A : 0.51 3D Compatibility (PKB) : 37.71 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.51 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_4GCZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GCZ-query.scw PDB file : Tito_Scwrl_4GCZ.pdb: