Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQIKIKYLDETQTRINKMEQGDWIDLRAAEDVAIKKDEFKLVPLGVAMELPEGYEAHVVPRSSTYKNFGVIQTNSMGVIDESYKGDNDFWFFPAYALRDTKIKKGDRICQFRIMKKMPAVDLIEVDRLGNGDRGGHGSTGTK 4B0H Chain:C ((3-144)) MQIKIKYLDETQTRISKIEQGDWIDLRAAEDVTIKKDEFKLVPLGVAMELPEGYEAHVVPRSSTYKNFGVIQTNSMGVIDESYKGDNDFWFFPAYALRDTEIKKGDRICQFRIMKKMPAVELVEVEHLGNEDRGGLGSTGTK

General information: TITO was launched using: RESULT: Template: 4B0H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 536 -65105 -121.46 -458.49 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain C : 0.99 3D Compatibility (PKB) : -121.46 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.99 QMean score : 0.453

(partial model without unconserved sides chains): PDB file : Tito_4B0H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4B0H-query.scw PDB file : Tito_Scwrl_4B0H.pdb: