Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRQVKCPYCETKLDKDSAI-PYKKRYYHEKCFNTWKQESDHRKELIQYICNLYGLTSPTGMMLKQIKEFQEEYGYKLK-----GIELALRYFYETLDNQPREGDGIGIVPFVYDEAKRHYI--RQKAIRKSAEDPK--NHKREEITLVIKKGMRKKRGLVDISML 4ZC4 Chain:A ((12-158)) -------QHPSHELLKENGFTQHVYHKYRRRCLNERKRLGIGQSQEMNTLFRFWSFFLRDHFNKKMYEEFKQLALEDAKEGYRYGLECLFRYYSYGLEKKFR----LDIFKDFQEETVKDYEAGQLYGLEKFWAFLKYSKAKNLDIDPKLQEYLGKFR--------

General information: TITO was launched using: RESULT: Template: 4ZC4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 512 24176 47.22 176.47 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 47.22 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.323

(partial model without unconserved sides chains): PDB file : Tito_4ZC4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZC4-query.scw PDB file : Tito_Scwrl_4ZC4.pdb: