Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MNNIGEIISNFEGIIGALL------GVIVTLILTHILKHFGQIKFYIVDFEIYFKTDNDGWGTNVMPSKDEAKQIEIH---------SQIEIYNGAEIP--------------KVLREIKFCFYKNTNLIVSV---TPDDKATTEEFA-EFGYYRDKLFNINLPSKQII---------AINIIKFL---NEKETKQVKKCNRVYLEAKDHNGKMYKVFLGEF------------------------ 2WFG Chain:A ((2-251)) GYVGIKIRLTDVAPQAQELFKKESLDVKENKVYLVAATLRPETMYGQTCCFVSPKIDYGVFDAGN-GDYFITTERAFKNMSFQNLTPKRGYYKPLFTINGKTLIGSRIDAPYAVNKNLRVLPMETVLATKGTGVVTCVPSDSPDDFVTTRDLANKPEYYGIEKDWVQTDIVPIVHTEKYGDKCAEFLVNDLKIQSPKDSVQLANAKELAYKEGFYNGTMLIGKYKGDKVEDAKPKVKQDLIDEGLAFVYNE

General information: TITO was launched using: RESULT: Template: 2WFG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 945 7520 7.96 42.72 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 7.96 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.144

(partial model without unconserved sides chains): PDB file : Tito_2WFG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WFG-query.scw PDB file : Tito_Scwrl_2WFG.pdb: