Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKWKRLFFILLAINFILAAGFVALVLLPGEQAQVKDASESEYGFQVTSTKESLAAFVNSYLNDKASNKLDYKVEIDDDVHVAGKIKAFSTSIDAFIAFE-----PTVKKNGD--VELNVTKFSLGKLSIPISFVLNYMDSFYELPSFVHVHPGDKSIEVRLSEMPLTNGMYVKADKINLEKDEIEFSYYHPKQ 2HX6 Chain:A ((1-153)) MTINTEVFIRRNKLRRHFESEFRQINNEIREASKAAGVS----SFHLKYSQALLDRAIQREIDET---------------YVFELFHKIKDHVLEVNEFLSMPPRPDIDEDFIDGVEYRPGRLEITDGNLWLGFTVCKPNEKFKDPSLQC-------RMAIINSRRLPGKASKAVIKTQ---------------

General information: TITO was launched using: RESULT: Template: 2HX6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 567 -28214 -49.76 -193.25 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -49.76 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.364

(partial model without unconserved sides chains): PDB file : Tito_2HX6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HX6-query.scw PDB file : Tito_Scwrl_2HX6.pdb: