Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKVFIKALPVLRILIEAGHQAYFVGGAVRDSYMKRTI---GDVDIATDAAPDQVERLFQRTVDVGKEH------G-------TIIVLWE----DETYEVTTFRTESDYVDFRRPSEVQFISSLEEDLKRRDLTINAMAMTAD---------GKVLDYFGGKKDIDQKVIRTVGKPEDRFQEDALRMLRAVRFMSQLGFTLSPETEEAIAKEKSLLSHVSVERKTIEFEKLLQGRASRQALQTLIQTRLYEELPGFYHKRENLISTSEFPF------------F----------------------------SLTSREELWAALL-INLGIVL------------KDAPLFLKAWKLPGKVIKEAIHIADT-FGQSL-----------DAMTMYRAGKKALLSAA---KISQLRQNEKLDEKKLKDIQYAYQNLPIKSLKDLDITGKDLLALRNRPAGKWVSEELQWIEQAVVTGKLSNQKKHIEEWLKTCGQH 3WFP Chain:A ((7-356)) -----SYFDDVAKVL-PREHYCFIVGGWVRDRILGEPVGYNIDVDFLTTADPVELAKNFAKR--IGGHFFVFEKRGFLIKRPTIASVVLHLPPYRYRFDFSPLKGK------------DLEKALIEDLKERDFTANAIAVNLDDVLSIGAKQTIVYDPTGGIKDLEQGLLRPVSI--ENLKRDPVRVLRGFRIAIEKNLQLTEDFYEFVKEDPRIVLKSAVERITHELFKIMKEKTAHKVIRELYEYGVLEAIIPEIGRLREVKD------PLDEHTLKTLEYLEQVIEDRAKYLSAELLENFGKKRVLGEFTDVELL-KWGALFHDIGKPQTFVTFYEHDKVGAQIVREIGERLRWGDEATEFVAKLVRHHL-RPFFLREAFKKGELKRRGMANFWRECGDIAPHLFLLSIADAMAS----------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3WFP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1159 15258 13.16 59.83 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 13.16 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.494

(partial model without unconserved sides chains): PDB file : Tito_3WFP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WFP-query.scw PDB file : Tito_Scwrl_3WFP.pdb: