Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEIAEVLPESMKNALSEIPEQQWLEIEEVRIRINRPVELIRRGQPVY-LSYAGTAEDAHLILSRLSNYSMY-TLEEE-LKKGYVTIRGGHRVGLAGRVITENGGVKGLRDIASFNIRIARQKLGIAEPLLPYLYQNSWLNTLIIGPPQTGKTTLLRDLARLSSTGKKNMLPVKTGIVDERSEIAGCLR--GIPQHQFGQRIDVLDACPKAEGLMMMIRSMSPEVMIVDEIGRMEDTDALLEALHAGVSVIVSAHGWSISDLMKRPSLKRLWEERAFDRYLELSRAKGPGTVSQIYDKDGNVLSRTTGVKTC 3JVV Chain:A ((2-256)) ----------DITELLAF---SAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLEHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVARFRVNAFNQN-RGAGAVFRTIPSKVLTM--EELGMGE-VFKRVSDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKY----HHILTIEDPIEFVHESKKCLVNQREVHR-----DTLGFSEALRSALR-EDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRVVDVFP-----------------------------------------

General information: TITO was launched using: RESULT: Template: 3JVV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1147 -155 -0.13 -0.65 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -0.13 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.306

(partial model without unconserved sides chains): PDB file : Tito_3JVV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3JVV-query.scw PDB file : Tito_Scwrl_3JVV.pdb: