Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTPSMEDYIEQIYMLIEEKGYARVSDIAEALAVHPSSVTKMVQKLDKDEYLIYEKYRGLVLTSKGKKIGKRLVYRHELLEQFLRIIGVDEEKIYNDVEGIEHHLSWNSIDRIGDLVQYFEEDDARKKDLKSIQKKTEHHNQ 2F5D Chain:A ((3-136)) --TPSMEDYIEQIYMLIEEKGYARVSDIAEALAVHPSSVTKMVQKLDKDEYLIY----GLVLTSKGKKIGKRLVYRHELLEQFLRIIGVDEEKIYNDVEGIEHHLSWNSIDRIGDLVQYFEEDDARKKDLKSIQKK------

General information: TITO was launched using: RESULT: Template: 2F5D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 409 -74963 -183.28 -576.64 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.98 3D Compatibility (PKB) : -183.28 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.98 QMean score : 0.637

(partial model without unconserved sides chains): PDB file : Tito_2F5D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F5D-query.scw PDB file : Tito_Scwrl_2F5D.pdb: