Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFTAYQDARSWIHGRLKFGVKPGLGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNERISVNGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIMTACAFLYFAEFHKVDFVIFETGLGGRFDSTNVVEPLLTVITSIGHDHMNILGNTIEEIAGEKAGIIKEG---IPIVTAVTQPEALQVIRHEAERHAAPFQSLHDAC-VIF-NEEALPAGEQFSFKTEEKCYED-IRTSLIGTHQRQNAALSILAAEWLNKENIAHISDEALRSGLVK-AAWPGRLELVQE-HPPVYLDGA--HNEEGVEKLAETMKQRFANSRISVVFSAL-KDKPYQNMIKRLETIAHAIHFASFDFPRASLAKDLYD---ASEISNKSWSEDPDDVIKFIESKKGSNEIVLITGSLYFISDIRKRLK 3LK7 Chain:A ((111-432)) ---------------------------------------ESQLIGITGSNGKTTTTTMIAEVLNAGG-QRGLLAGNI-----------GFPA---------------------------S--EVV-------QAAN-DKDTLVMELSSFQLM-GVKEFRPHIAVITNLMPTHLDYHG-SFEDYVAAKWNIQNQMSSSDFLVLNFNQGISKE-LAKT---TKATIVPFSTTEKVDGAYVQ---DK-QLFYK--GENIMSVDDIGVPGSHNVENALATIAVAKLAG------ISNQVIRETLSNFGGVKHRLQSLGKVHGISFYNDSKSTNILATQKALSGFD----NTKVILIAGGLDRGNEFDELIPDITG-LKHMVVLGESA---SRVKRAAQKAGVTYSDALDVRDAVHK----AYEVAQQGDVILLSPANASWDMYK----

General information: TITO was launched using: RESULT: Template: 3LK7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1639 -35125 -21.43 -114.04 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -21.43 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.399

(partial model without unconserved sides chains): PDB file : Tito_3LK7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LK7-query.scw PDB file : Tito_Scwrl_3LK7.pdb: