Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKHTFVLFLSLILLVLPGCSAEKSSADTAKKTLTIYSTMSTDSERDTFRKLAAAFEKEHSDIHVSLHF-PGNDYENMMRVRMAANDLPDLFDTHG---WGKIRYGEYTADLRDMKW---TQDLDPNLNSILKNKSGKVYAYPINQAKDGLAYNRNILDRYGIAPPETMDDFIKALRTIKEKSKGSIVPFWFAGYDKSSFAQYYDQFATPLLITDPAHNEKKQLINGTFQW-S-KFTYLSEILKQMQKEKLINIDAVTAKKSQLIELMAQNKIAFTMQGGTLGQDVAQIN---PNVKVGIIPTPAIHPGDD--PIWIGGERYTLAAWKDSPQLKEAKDFIAFMARPANAKQMAEATSLPSGLTNVKADIFYANDY---E-YYQDVKVE-PYFDRLYLPNG-MWD-VLGTVGQELAADILAPQDISQKLGREYKRLREQSETQGAENNE 4G68 Chain:A ((63-454)) ------------------------------ITLTFWNLFTGEPAKTKVKEIIDQWNKENPNVQIVESVTENDAYKTKIKAAIAANEAPDIFQTWAGGFSQPFVEAGKVLQLDSYLNDGTKDQLLPGSFDN-VTYNGKIYGIPFDQQASVLYINKELFDKYNVKVPTTFSELIDAIKTFK-S-KG-VTPFALGEKDEWPGMWYYDMIALRE----GGVQLTRDALNGKASFDNQAFTDAAQKLQDMVNAGAFDSGFMGLTRDEATAEFNQGKAAMYFGGNFDAAAFVSDPSSLVKGKIEAVRFPTIEGGKGDPTEYIGGTVGALMVSANSKYKDEAVRAAKYLAKQLSDMDYLIAT-GLPAWKYDNIDQ-SKVDPLEIQIMNNIVANAKGSVPAWDIYLSGDAAQTHKDLVAQLFAKQITPEEYSKQMQQKIN---------------

General information: TITO was launched using: RESULT: Template: 4G68.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2119 68213 32.19 183.86 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 32.19 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.398

(partial model without unconserved sides chains): PDB file : Tito_4G68.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G68-query.scw PDB file : Tito_Scwrl_4G68.pdb: