Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVTVREAKLEDIKDIAKVHVDSWRTTYHEIIPIDYLNSLNYKEFEDKWKSRSLKGVFVAQDE-------KGSVFGFASFGPIRSEQEGYDGELYAIYLLEERQRQGAGRALLAKGAEFLLQHGFSSMFVWVIEQN-PSIIFYQAYSPERVAEDNFEIAGVRLKEVGLGWPDLSALKTLLNR 2B5G Chain:A ((3-151)) KFVIRPATAADCSDILRLIKELAKYE----QVI---LT-EKDLLEDGFGEHPFYHCLVAEVPKEHWTPEGHSIVGFAMYYFTYDPWIGKLLYLEDFFVMSDYRGFGIGSEILKNLSQVAMRCRCSSMHFLVAEWNEPSINFYKRRGASDLSSE----------------------------

General information: TITO was launched using: RESULT: Template: 2B5G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 519 -19907 -38.36 -145.31 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -38.36 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.424

(partial model without unconserved sides chains): PDB file : Tito_2B5G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2B5G-query.scw PDB file : Tito_Scwrl_2B5G.pdb: